Response of acetylcholine receptors to photoisomerizations of bound agonist molecules.

@article{Nass1978ResponseOA,
  title={Response of acetylcholine receptors to photoisomerizations of bound agonist molecules.},
  author={Menasche M. Nass and Henry A. Lester and Mauri E. Krouse},
  journal={Biophysical journal},
  year={1978},
  volume={24 1},
  pages={135-60}
}
In these experiments, agonist-induced conductance is measured while a sudden perturbation is produced at the agonist-receptor binding site. A voltage-clamped Electrophorus electroplaque is exposed to trans-Bis-Q, a potent agonist. Some channels are open; these receptors have bound agonist molecules. A light flash isomerizes 3(-35)% of the trans-Bis-Q molecules to their cis form, a far poorer agonist. This causes a rapid decrease of membrane conductance (phase 1), followed by a slower increase… CONTINUE READING