Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells

@article{Uster1986ResonanceET,
  title={Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells},
  author={Paul S. Uster and Richard E. Pagano},
  journal={The Journal of Cell Biology},
  year={1986},
  volume={103},
  pages={1221 - 1234}
}
A conventional fluorescence microscope was modified to observe the sites of resonance energy transfer (RET) between fluorescent probes in model membranes and in living cells. These modifications, and the parameters necessary to observe RET between membrane-bound fluorochromes, are detailed for a system that uses N-4-nitrobenzo-2-oxa-1,3-diazole (NBD) or fluorescein as the energy donor and sulforhodamine as the energy acceptor. The necessary parameters for RET in this system were first optimized… CONTINUE READING
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Applications of digitized fluorescence microscopy to problems in cell biology• Biotechniques

  • J. DiGuiseppi, Inman, A. Ishihara, K. Jacobson, B. Herman
  • 1985
3 Excerpts

Fluorescence energy transfer between cobra or - toxin molecules bound to the acetytcholine receptor

  • L. V. Johnson, M. L. Walsh, B. J. Bockus, L. B. Chen
  • J . Biol . Chem .
  • 1984

Transport of a fluorescent phosphatidylcholine analog from the plasma membrane to the Golgi apparatus

  • D. K. Struck, D. Hoekstra, R. E. Pagano
  • J . Cell BioL
  • 1984

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