Resolving single-molecule assembled patterns with superresolution blink-microscopy.

  title={Resolving single-molecule assembled patterns with superresolution blink-microscopy.},
  author={Thorben Cordes and Mathias Strackharn and Stefan W Stahl and Wolfram Summerer and Christian Steinhauer and Carsten Forthmann and Elias M. Puchner and Jan Vogelsang and Hermann Eduard Gaub and Philip Tinnefeld},
  journal={Nano letters},
  volume={10 2},
In this paper we experimentally combine a recently developed AFM-based molecule-by-molecule assembly (single-molecule cut-and-paste, SMCP) with subdiffraction resolution fluorescence imaging. Using "Blink-Microscopy", which exploits the fluctuating emission of single molecules for the reconstruction of superresolution images, we resolved SMCP assembled structures with features below the diffraction limit. Artificial line patterns then served as calibration structures to characterize parameters… CONTINUE READING

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