Resolving individual steps of Okazaki fragment maturation at msec time-scale

@inproceedings{Stodola2016ResolvingIS,
  title={Resolving individual steps of Okazaki fragment maturation at msec time-scale},
  author={Joseph L. Stodola and Peter M. J. Burgers},
  booktitle={Nature Structural &Molecular Biology},
  year={2016}
}
DNA polymerase delta (Pol δ) is responsible for elongation and maturation of Okazaki fragments. Pol δ and the flap endonuclease FEN1, coordinated by the PCNA clamp, remove RNA primers and produce ligatable nicks. We studied this process in the Saccharomyces cerevisiae machinery at millisecond resolution. During elongation, PCNA increased the Pol δ catalytic rate by >30-fold. When Pol δ invaded double-stranded RNA–DNA representing unmatured Okazaki fragments, the incorporation rate of each… CONTINUE READING