Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining.

@article{Grgori2001ResolutionOV,
  title={Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining.},
  author={G{\'e}rald Gr{\'e}gori and Sandra Citterio and Alessandra Ghiani and Massimo Labra and Sergio Sgorbati and Steve S Brown and Michel Denis},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 10},
  pages={4662-70}
}
The membrane integrity of a cell is a well-accepted criterion for characterizing viable (active or inactive) cells and distinguishing them from damaged and membrane-compromised cells. This information is of major importance in studies of the function of microbial assemblages in natural environments, in order to assign bulk activities measured by various methods to the very active cells that are effectively responsible for the observations. To achieve this task for bacteria in freshwater and… CONTINUE READING