Resolution of three structural states of spin-labeled myosin in contracting muscle.

@article{Ostap1995ResolutionOT,
  title={Resolution of three structural states of spin-labeled myosin in contracting muscle.},
  author={E Michael Ostap and Vincent A. Barnett and David D Thomas},
  journal={Biophysical journal},
  year={1995},
  volume={69 1},
  pages={177-88}
}
We have used electron paramagnetic resonance (EPR) spectroscopy to detect ATP- and calcium-induced changes in the structure of spin-labeled myosin heads in glycerinated rabbit psoas muscle fibers in key physiological states. The probe was a nitroxide iodoacetamide derivative attached selectively to myosin SH1 (Cys 707), the conventional EPR spectra of which… CONTINUE READING