Resolution of mitochondrial and chloroplast membrane protein complexes from green leaves of potato on blue-native polyacrylamide gels.

@article{Singh2000ResolutionOM,
  title={Resolution of mitochondrial and chloroplast membrane protein complexes from green leaves of potato on blue-native polyacrylamide gels.},
  author={Pawan K Singh and Lothar J{\"a}nsch and Hans Peter Braun and Udo Klaus Schmitz},
  journal={Indian journal of biochemistry & biophysics},
  year={2000},
  volume={37 1},
  pages={59-66}
}
Purification of mitochondria and mitochondrial protein complexes from green tissues is often severely impaired by the presence of chloroplasts and their proteins. Here we present a method which allows analysis of respiratory protein complexes from potato leaves. The procedure includes the preparation of an organellar fraction specifically enriched in mitochondria and the separation of organellar protein complexes by blue-native polyacrylamide gel electrophoresis (BN-PAGE). For the first time… CONTINUE READING