Resolution of complex anisotropy decays by variable frequency phase-modulation fluorometry: a stimulation study.

We used simulations to determine the resolution of complex anisotropy decay laws which is obtainable by frequency-domain fluorometry. The simulations include the effects of torsional and segmental motions of tryptophan residues in proteins, the multiple correlation times of asymmetric molecules, and three-component anisotropy decays. For a protein with a… CONTINUE READING