Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels

  title={Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels},
  author={M. Prinz and C. Schmitt and M. Staak and H. Baum},
Utilizing three different electrophoresis systems for the separation of variable number of tandem repeats (VNTR) alleles of the high variable region 3′ from the apolipoprotein B (Apo B) locus, it could be shown that apparent allele subtypes on native polyacrylamide gels comigrated with the regular ladder alleles on agarose and denaturing polyacrylamide gels. Two subtypes, one with a shift towards the cathode and one shifted towards the anode, and the corresponding ladder alleles were sequenced… Expand
Typing dinucleotide repeats under nondenaturing conditions with single-base resolution and high sizing precision
This PAGE system is thus also useful for reducing the likelihood both of allele misidentification due to the absence of single-base resolution and of inaccuracies in allele sizing due to anomalous electrophoretic migrations among the alleles within an (AC)n repeat. Expand
Allele frequency distributions of six Amp-FLPS (D1S80, APO-B, VWA, TH01, CSF1PO and HPRTB) in a Mexican population.
Six amplified fragment length polymorphisms or Amp-FLPs, two VNTRs and APO-B and four STRs were typed in a Mexican population of the Jalisco state by means of non-denaturing polyacrylamide gel electrophoresis (native PAGE) in standard gel units and silver staining. Expand


Nucleotide sequence analysis of the apolipoprotein B 3' VNTR.
The data suggest that polarized variability may be independent of the mutational process(es) generating length variation at minisatellite loci and suggest a possible alternative mechanism of mutation that involves the formation of secondary structures. Expand
Rapid typing of tandemly repeated hypervariable loci by the polymerase chain reaction: application to the apolipoprotein B 3' hypervariable region.
The high sensitivity and inexpensive nature of this technique make it superior to traditional Southern blot analysis for typing the 3' apoB VNTR and directly applicable for rapid typing of other VN TRs in the human genome. Expand
High-resolution analysis of a hypervariable region in the human apolipoprotein B gene.
A hypervariable region occurs immediately 3' of the human apolipoprotein B gene. Several allelic variants of this tandemly repeated sequence can be resolved by genomic blotting. Higher resolutionExpand
A unique AT-rich hypervariable minisatellite 3' to the ApoB gene defines a high information restriction fragment length polymorphism.
The heterogeneity of this AT-rich minisatellite provides the basis for a highly informative restriction fragment length polymorphism of the apoB gene and should be very useful in association and linkage analysis studies of the contribution of this locus to atherosclerosis susceptibility. Expand
Variant mapping of the Apo(B) AT rich minisatellite. Dependence on nucleotide sequence of the copy number variations. Instability of the non-canonical alleles.
The sharp analysis of the minisatellite structure by the distribution of SspI sites reveals differences between long and short alleles, indicating that in most cases, no recombination occurs between alleles of different sizes and the rare alleles exhibit a non-canonical structure. Expand
DNA length polymorphism of the apo B 3' region: frequency distribution of the alleles in the German population.
The allele distribution of 340 unrelated individuals originating predominantly from Southern Germany was studied, finding that at least 16 different alleles can be distinguished. Expand
Sequence-directed curvature of DNA.
An electrophoretic analysis of a series of closely related DNA polymers in which oligo(dA)-oligo(dT) runs of different polarity were compared concluded that ApA dinucleotide wedges cannot account for DNA curvature. Expand
Amplification of the hypervariable region close to the apolipoprotein B gene: application to forensic problems.
By comparing the fragments produced from the stains with those from the blood samples the authors were able to identify the origin of the blood stains in both cases. Expand
High sensitive DNA typing approaches for the analysis of forensic evidence: comparison of nested variable number of tandem repeats (VNTR) amplification and a short tandem repeats (STR) polymorphism.
The approach of using nested primers for the APO B variable number of tandem repeats (VNTR) increases the sensitivity of the polymerase chain reaction (PCR) to single cell level and short tandem repeats system VWA showed less preferential amplification. Expand
Automated DNA profiling by fluorescent labeling of PCR products.
DNA profiling has been automated by the fluorescent tagging of amplified variable number tandem repeat (VNTR) loci. This was achieved by the use of fluorescently labeled primers in the amplificationExpand