Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels

@article{Prinz1996ResolutionOA,
  title={Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels},
  author={M. Prinz and C. Schmitt and M. Staak and H. Baum},
  journal={ELECTROPHORESIS},
  year={1996},
  volume={17}
}
Utilizing three different electrophoresis systems for the separation of variable number of tandem repeats (VNTR) alleles of the high variable region 3′ from the apolipoprotein B (Apo B) locus, it could be shown that apparent allele subtypes on native polyacrylamide gels comigrated with the regular ladder alleles on agarose and denaturing polyacrylamide gels. Two subtypes, one with a shift towards the cathode and one shifted towards the anode, and the corresponding ladder alleles were sequenced… Expand
Typing dinucleotide repeats under nondenaturing conditions with single-base resolution and high sizing precision
TLDR
This PAGE system is thus also useful for reducing the likelihood both of allele misidentification due to the absence of single-base resolution and of inaccuracies in allele sizing due to anomalous electrophoretic migrations among the alleles within an (AC)n repeat. Expand
Allele frequency distributions of six Amp-FLPS (D1S80, APO-B, VWA, TH01, CSF1PO and HPRTB) in a Mexican population.
TLDR
Six amplified fragment length polymorphisms or Amp-FLPs, two VNTRs and APO-B and four STRs were typed in a Mexican population of the Jalisco state by means of non-denaturing polyacrylamide gel electrophoresis (native PAGE) in standard gel units and silver staining. Expand

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