Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the recombination site

@article{Rogowsky1984ResolutionOA,
  title={Resolution of a hybrid cointegrate between transposons Tn501 and Tn1721 defines the recombination site},
  author={Peter M Rogowsky and Ruediger Schmitt},
  journal={Molecular and General Genetics MGG},
  year={1984},
  volume={193},
  pages={162-166}
}
The related transposons Tn501 and Tn1721 have a 3.8 kb region in common that contains two genes (tnpA and tnpR) and a resolution site (res) required for transposition. Resolvase, the product of tnpR, catalyses site-specific recombination at res, a 186 base pair (bp) sequence located adjacent to tnpR at one end of the homology region. We describe here identification of the crossover site within res. It involved the construction of a plasmid containing copies of res (Tn501) and res (Tn1721) in… CONTINUE READING

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