Resolution and properties of the catalytic component and phosphate acceptor proteins of a new protein kinase-substrate complex from Morris hepatoma 3924A.

@article{Criss1978ResolutionAP,
  title={Resolution and properties of the catalytic component and phosphate acceptor proteins of a new protein kinase-substrate complex from Morris hepatoma 3924A.},
  author={Wayne E. Criss and Masaki Yamamoto and Yoshimi Takai and Yasutomi Nishizuka and Harold P. Morris},
  journal={Cancer research},
  year={1978},
  volume={38 10},
  pages={3540-5}
}
A self-phosphorylating, polylysine-stimulated protein kinase, which was partially purified from the cytosol of fastgrowing Morris hepatoma 3924A, was resolved into a catalytic component and several phosphate acceptor pro teins when subjected to a phosphocellulose column. Other Chromatographie and electrophoretic procedures were unable to dissociate them. When once dissociated, the catalytic component phosphorylated preferentially ca sein and nuclear phosphoproteins but not histone and protamine… CONTINUE READING