Resolution and kinetic characterization of glutathione S-transferases from human jejunal mucosa.

Abstract

Cytosolic glutathione S-transferases were purified from human jejunal mucosa by affinity chromatography on S-hexylglutathione-Sepharose 4B. Chromatofocusing in the pH range 7-4 yielded peaks with apparent pI's of 7.2 (peak 1), 5.2 (peak 2), and 4.4 (peak 3). Each enzymatic fraction was shown to have a homodimeric structure, with subunit mass of 24.9 +/- 0.5… (More)

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@article{Ozer1990ResolutionAK, title={Resolution and kinetic characterization of glutathione S-transferases from human jejunal mucosa.}, author={Nilgiin Ozer and Ozge Erdemli and Iskender Sayek and Inci Ozer}, journal={Biochemical medicine and metabolic biology}, year={1990}, volume={44 2}, pages={142-50} }