Resolution and identification of O-phosphoserine, O-phosphothreonine, O-phosphotyrosine, and γ-carboxyglutamic acid as their fluorescent o-phthalaldehyde derivatives by high performance liquid chromatography

Abstract

High performance liquid chromatography was used to resolve O-phosphoserine, O-phosphothreonine, and O-phosphotyrosine as their fluorescent o-phthalaldehyde derivatives. By adjusting the buffer system, very small amounts of O-phosphothreonine could be detected and quantitated in the presence of very large amounts of O-phosphoserine. In addition… (More)
DOI: 10.1007/BF02555211

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