Resolution and characterisation of multiple isoforms of bovine kappa-casein by 2-DE following a reversible cysteine-tagging enrichment strategy.

@article{Holland2006ResolutionAC,
  title={Resolution and characterisation of multiple isoforms of bovine kappa-casein by 2-DE following a reversible cysteine-tagging enrichment strategy.},
  author={J. William Holland and Hilton C Deeth and Paul F Alewood},
  journal={Proteomics},
  year={2006},
  volume={6 10},
  pages={3087-95}
}
Visualisation of multiple isoforms of kappa-casein on 2-D gels is restricted by the abundant alpha- and beta-caseins that not only limit gel loading but also migrate to similar regions as the more acidic kappa-casein isoforms. To overcome this problem, we took advantage of the absence of cysteine residues in alpha(S1)- and beta-casein by devising an… CONTINUE READING