Resolution and Recovery of Large Dna Fragments

  title={Resolution and Recovery of Large Dna Fragments},
  author={Daniel F. Voytas},
This section describes the application of agarose gel electrophoresis to both analytical and preparative separation of DNA fragments. Standard agarose gels separate DNA fragments from ∼0.5 to 25 kb, whereas pulsed-field agarose gels resolve molecules from ∼10 to >2000 kb. Descriptions of standard and pulsed-field agarose gel electrophoresis as well as parameters affecting resolution of large DNA fragments are presented in UNITS 2.5A and 2.5B. Three different protocols that employ agarose gels… CONTINUE READING