Resistance to Pediococcus cerevisiae to amethopterin as a consequence of changes in enzymatic activity and cell permeability. II. Permeability changes to amethopterin and other folates in the drug-resistant mutant.

@article{MandelbaumShavit1976ResistanceTP,
  title={Resistance to Pediococcus cerevisiae to amethopterin as a consequence of changes in enzymatic activity and cell permeability. II. Permeability changes to amethopterin and other folates in the drug-resistant mutant.},
  author={F. Mandelbaum-Shavit},
  journal={Biochimica et biophysica acta},
  year={1976},
  volume={428 3},
  pages={
          674-82
        }
}
  • F. Mandelbaum-Shavit
  • Published 1976
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • the accumulation of amethopterin in a Pediococcus cerevisiae strain resistant to this analogue was about 30% of that in P. cerevisiae/PteGlu, the sensitive parent. The uptake in the resistant strain was strictly glucose dependent, whereas in the sensitive parent about 16% accumulation occurred in absence of glucose. The transport in both strains was inhibited by iodoacetate and KF. Amethopterin uptake exhibited saturation kinetics with an apparent Km of 5 muM in P. cerevisiae/AMr and 0.5 muM in… CONTINUE READING
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