Resistance in a laboratory population of Culex quinquefasciatus (Diptera: Culicidae) to Bacillus sphaericus binary toxin is due to a change in the receptor on midgut brush-border membranes.

@article{NielsenLeroux1995ResistanceIA,
  title={Resistance in a laboratory population of Culex quinquefasciatus (Diptera: Culicidae) to Bacillus sphaericus binary toxin is due to a change in the receptor on midgut brush-border membranes.},
  author={Christina Nielsen-Leroux and J-P. Charles and Isabelle Thi{\'e}ry and George P. Georghiou},
  journal={European journal of biochemistry},
  year={1995},
  volume={228 1},
  pages={
          206-10
        }
}
Direct binding experiments with isolated brush border membrane fractions (BBMF) from larvae of a susceptible laboratory strain of Culex quinquefasciatus Say, indicated the presence of a single class of Bacillus sphaericus binary toxin receptors. The dissociation constant (Kd) was approximately 11 nM and the maximum binding capacity (Bmax) approximately 8 pmol/mg BBMF protein. Similar binding experiments with a field population of C. quinquefasciatus that had been selected in the laboratory to… CONTINUE READING
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