Residues in the RNP1-like sequence motif of Rho protein are involved in RNA-binding affinity and discrimination.

@article{Martinez1996ResiduesIT,
  title={Residues in the RNP1-like sequence motif of Rho protein are involved in RNA-binding affinity and discrimination.},
  author={Aurora Martinez and Christopher M. Burns and John P. Richardson},
  journal={Journal of molecular biology},
  year={1996},
  volume={257 5},
  pages={909-18}
}
The termination of transcription in Escherichia coli by action of Rho factor is dependent on the ability of this homohexameric protein to make productive interactions with the nascent RNA molecule to be terminated. The roles of two residues in a phylogenetically conserved sequence motif in the RNA-binding domain of Rho, Asp60 and Phe62, were analyzed by studies of the biochemical properties of pure mutant proteins. F62S Rho had greatly reduced affinity for lambda cro RNA, very poor ability to… CONTINUE READING

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