Residues Controlling Facial Selectivity in an Alkene Reductase and Semirational Alterations to Create Stereocomplementary Variants

@inproceedings{Walton2014ResiduesCF,
  title={Residues Controlling Facial Selectivity in an Alkene
Reductase and Semirational Alterations to Create Stereocomplementary
Variants},
  author={Adam Z. Walton and Bradford Sullivan and Ath{\'e}na C. Patterson-Orazem and Jon D. Stewart},
  booktitle={ACS catalysis},
  year={2014}
}
A systematic saturation mutagenesis campaign was carried out on an alkene reductase from Pichia stipitis (OYE 2.6) to develop variants with reversed stereoselectivities. Wild-type OYE 2.6 reduces three representative Baylis-Hillman adducts to the corresponding S products with almost complete stereoselectivities and good catalytic efficiencies. We created and screened 13 first-generation, site-saturation mutagenesis libraries, targeting residues found near the bound substrate. One variant… CONTINUE READING

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