Residue 21 of human granulocyte-macrophage colony-stimulating factor is critical for biological activity and for high but not low affinity binding.

@article{Lopez1992Residue2O,
  title={Residue 21 of human granulocyte-macrophage colony-stimulating factor is critical for biological activity and for high but not low affinity binding.},
  author={Angel F Lopez and M. Frances Shannon and Timothy R. Hercus and Nicos A. Nicola and Bronwyn A Cambareri and Mara Dottore and Meredith J. Layton and Lisa K. Eglinton and Mathew A. Vadas},
  journal={The EMBO journal},
  year={1992},
  volume={11 3},
  pages={909-16}
}
The functional role of the predicted first alpha-helix of human granulocyte-macrophage colony-stimulating factor (GM-CSF) was analysed by site-directed mutagenesis and multiple biological and receptor binding assays. Initial deletion mutagenesis pointed to residues 20 and 21 being critical. Substitution mutagenesis showed that by altering Gln20 to Ala full GM-CSF activity was retained but that by altering Glu21 for Ala GM-CSF activity and high affinity receptor binding were decreased… CONTINUE READING

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