Resident microbiota of the gypsy moth midgut harbors antibiotic resistance determinants.

  title={Resident microbiota of the gypsy moth midgut harbors antibiotic resistance determinants.},
  author={Heather K. Allen and Karen A. Cloud-Hansen and Joseph M Wolinski and Changhui Guan and Serena Greene and Shyue Lu and Mallory Boeyink and Nichole A Broderick and Kenneth F Raffa and J. C. Handelsman},
  journal={DNA and cell biology},
  volume={28 3},
Little is known about the significance of insects as environmental reservoirs of antibiotic-resistant bacteria. We characterized the antibiotic resistome of the microbial community in gypsy moth larval midguts by applying functional metagenomics to cultured isolates. The minimum inhibitory concentrations of 12 antibiotics were determined for 44 cultured isolates, and antibiotic resistance genes were selected from metagenomic libraries derived from DNA extracted from a pool of the isolates. Six… CONTINUE READING
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