Research resources for Drosophila: the expanding universe

  title={Research resources for Drosophila: the expanding universe},
  author={K. Matthews and Thomas C. Kaufman and William M. Gelbart},
  journal={Nature Reviews Genetics},
Drosophila melanogaster has been the subject of research into central questions about biological mechanisms for almost a century. The experimental tools and resources that are available or under development for D. melanogaster and its related species, particularly those for genomic analysis, are truly outstanding. Here we review three types of resource that have been developed for D. melanogaster research: databases and other sources of information, biological materials and experimental… 

Transgenesis upgrades for Drosophila melanogaster

These new approaches will greatly facilitate the structure-function analyses of Drosophila genes, will enhance the ease and speed with which flies can be manipulated, and should advance the understanding of biological processes during normal development and disease.

Drosophila melanogaster: a case study of a model genomic sequence and its consequences.

The impact of the growing number of genome sequences now available in the genus on current Drosophila research, and some of the biological questions that these resources will enable to be solved in the future are discussed.

Genetics on the Fly: A Primer on the Drosophila Model System

This primer describes the organism’s natural history, the features of sequenced genomes within the genus, the wide range of available genetic tools and online resources, the types of biological questions Drosophila can help address, and historical milestones.

Drosophila Genetic Resource and Stock Center; The National BioResource Project.

The history of Drosophila research is described and a few examples of the application of the abundant genetic resources of Dosophila to basic biology and medical investigations are provided.

Drosophila Polymorphism Database (DPDB)A Portal for Nucleotide Polymorphism in Drosophila

The state-of-the-art of the DPDB database is described and a step-by-step guide to all its searching and analytic capabilities is provided to illustrate its usefulness.

Exploring prion protein biology in flies

A Drosophila model of sporadic prion pathology that shares relevant features with the typical disease in mammals is reported, providing the basis to explore relevant aspects of the biology of the prion protein, such as uncovering the genetic mechanisms regulating prionprotein misfolding and prion-induced neurodegeneration in a dynamic, genetically tractable in vivo system.

Purification of Transcripts and Metabolites from Drosophila Heads

The procedures for collecting thousands of fly heads and the extraction of transcripts and metabolites to understand how global changes in gene expression and metabolism contribute to neurodegenerative diseases are described.

Data Pushing: A Fly-Centric Guide to Bioinformatics Tools

A broad overview of common bioinformatics tasks and the resources used to do them, with a specific focus on resources for Drosophila.

Transgenictools for members of the genus Drosophila with sequenced genomes

A set of guidelines and basic tools are provided for both insertional mutagenesis and GAL4/UAS system-based experiments in multiple species of Drosophila, demonstrating the possible need to use species-specific promoters in some cases.



Emerging technologies for gene manipulation in Drosophila melanogaster

Recent technologies that will allow Drosophila melanogaster to be manipulated more easily than any other multicellular organism are reviewed.

Drosophila, the golden bug, emerges as a tool for human genetics

  • E. Bier
  • Biology
    Nature Reviews Genetics
  • 2005
Drosophila melanogaster is emerging as one of the most effective tools for analyzing the function of human disease genes, including those responsible for developmental and neurological disorders,

The genome sequence of Drosophila melanogaster.

The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.

The Genetics and Biology of Drosophila

This is the first attempt since 1925 to publish a comprehensive account of the biology and genetics of Drosophila and it aims to collate the dauntingly large literature on the subject and to make more accessible the private language ot the Dosophilist.

FlyBase: genes and gene models

FlyBase ( is the primary repository of genetic and molecular data of the insect family Drosophilidae. For the most extensively studied species, Drosophila melanogaster, a wide

A Drosophila complementary DNA resource.

A general strategy for producing nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans is described, which so far has generated a set of cDNAs corresponding to over 40% of the genes in the fruit fly Drosophila melanogaster.

A Protein Interaction Map of Drosophila melanogaster

This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans, and recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components.

A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac

It is found that piggyBac has distinct global and local gene-tagging behavior from that of P elements, and is more effective at gene disruption because it lacks the P bias for insertion in 5′ regulatory sequences.

GAL4 system in drosophila: A fly geneticist's swiss army knife

The GAL4/UAS system in Drosophila is reviewed and the numerous extensions that have morphed it into a veritable Swiss army knife for the analysis of gene function are reviewed.