[Research on the protein C-activating property and influencial factors of protein C activator isolated from the venom of Agkistrodon halys].

Abstract

OBJECTIVE To investigate the protein C-activating property and experimental conditions that may affect the activities of protein C activator (PCA-SV). METHODS PCA-SV's functional characteristic, possible anticoagulant and amidase activities were measured by method of APTT and that of specific chromogenic substrate respectively in various experimental conditions. RESULTS PCA-SV can specifically act on protein C. The pH-optimum and temperature-optimum for the action of PCA-SV were 6.0 - 7.0 and 37 - 40 degrees C respectively. The anticoagulants (heparin, sodium citrate) had no effect on the activities. CONCLUSION PCA-SV is a new protein C-activating reagent that has high potency and specificity.

Cite this paper

@article{Hou2005ResearchOT, title={[Research on the protein C-activating property and influencial factors of protein C activator isolated from the venom of Agkistrodon halys].}, author={Liqiang Hou and Luning Zhao and Zhongxi Zack Zhao and Shaoling Huang and Quanhua He and Tianahn Kong and Jinxia Guan}, journal={Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials}, year={2005}, volume={28 3}, pages={198-201} }