Research of total levels on DNA methylation in plant based on HPLC analysis

  title={Research of total levels on DNA methylation in plant based on HPLC analysis},
  author={Qinghai Chen and Siyuan Tao and Xiaohua Bi and Xin Xu and LanLan Wang and Xue-Mei Li},
HPLC analysis is important for determination of total level on DNA methylation in plants. It can be used to help characterise epigenetic changes during growth, development and stress. HPLC methods have been optimised for mammalian and microbial DNA, but not for plants. This article examines several important factors in the HPLC analysis of plant DNA methylation including extraction and purification of DNA and HPLC conditions choice by using leaves of rice seedling. The experimental… CONTINUE READING


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