Rescue of shoot apical meristems of chrysanthemum by culturing on root tips

@article{Hosokawa2004RescueOS,
  title={Rescue of shoot apical meristems of chrysanthemum by culturing on root tips},
  author={Munetaka Hosokawa and Ayaka Otake and Yukihiro Sugawara and Toshimitsu Hayashi and Susumu Yazawa},
  journal={Plant Cell Reports},
  year={2004},
  volume={22},
  pages={443-448}
}
A new method to regenerate plants from leaf primordia-free shoot apical meristem domes (LP-free SAMs) was developed by establishing the meristem dome on the cut surface of root tips. Ten days after culture, the viable rate of LP-free SAMs of chrysanthemum ‘Piato’ attached to chrysanthemum root tips was >40%. Shoot regeneration was not observed from LP-free SAMs without the root tips. When LP-free SAMs of chrysanthemum were transferred to root tips of either petunia, cabbage, or carnation, the… CONTINUE READING

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