Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.

@article{Diener1990RescueOA,
  title={Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.},
  author={Dennis R. Diener and A. Malcolm Curry and Kristen A Johnson and Brett D. Williams and Paul A Lefebvre and Karen L. Kindle and Joel L. Rosenbaum},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 15},
  pages={
          5739-43
        }
}
The biflagellate alga Chlamydomonas has been used extensively in the genetic and biochemical analysis of flagellar assembly and motility. We have restored motility to a paralyzed-flagella mutant of Chlamydomonas by transforming with the corresponding wild-type gene. A nitrate reductase-deficient paralyzed-flagella strain, nit1-305 pf-14, carrying mutations in the genes for nitrate reductase and radial spoke protein 3, was transformed with wild-type copies of both genes. Two-thirds of the cells… CONTINUE READING

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