Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30

  title={Rescue of Recombinant Marburg Virus from cDNA Is Dependent on Nucleocapsid Protein VP30},
  author={Sven Enterlein and Viktor E. Volchkov and Michael Weik and Larissa Kolesnikova and Valentina A. Volchkova and Hans Dieter Klenk and Elke M{\"u}hlberger},
  journal={Journal of Virology},
  pages={1038 - 1043}
ABSTRACT Here we report recovery of infectious Marburg virus (MARV) from a full-length cDNA clone. Compared to the wild-type virus, recombinant MARV showed no difference in terms of morphology of virus particles, intracellular distribution in infected cells, and growth kinetics. The nucleocapsid protein VP30 of MARV and Ebola virus (EBOV) contains a Zn-binding motif which is important for the function of VP30 as a transcriptional activator in EBOV, whereas its role for MARV is unclear. It has… 
Phosphorylated VP30 of Marburg Virus Is a Repressor of Transcription
The largest outbreak of MARV occurred in Angola in 2004 to 2005 and had a 90% case fatality rate. There are no approved treatments available for MARV. Development of antivirals as therapeutics
Establishment and application of an infectious virus-like particle system for Marburg virus.
The infectious VLP system was demonstrated to be suitable for rapid screening of neutralizing antibodies directed against MARV and showed the highest capacity to induce reporter activity upon infection of target cells.
Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread.
R rescue of a recombinant Marburg virus expressing EGFP from an additional transcription unit (ATU) will be a useful tool not only to monitor virus spread and screen for antiviral compounds, but also to investigate the biology of inclusion body formation.
Ebola virus proteins NP, VP35, and VP24 are essential and sufficient to mediate nucleocapsid transport
A live-cell imaging system based on the ectopic expression of fluorescent Ebola virus (EBOV) fusion proteins is established, allowing the visualization and analysis of the movement of EBOV nucleocapsid-like structures with different protein compositions.
The Marburg Virus 3′ Noncoding Region Structurally and Functionally Differs from That of Ebola Virus
Differences in the structure of the genomic replication promoters might account for the different transcription strategies of Marburg and Ebola viruses.
Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
A model where the eVP30–eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy is supported.
Establishment of a Nipah virus rescue system
The rescued NiV (rNiV), by using the newly developed reverse genetics system, showed properties in vitro that were similar to the parent virus and retained the severe pathogenicity in a previously established animal model by experimental infection.


Comparison of the Transcription and Replication Strategies of Marburg Virus and Ebola Virus by Using Artificial Replication Systems
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Three of the Four Nucleocapsid Proteins of Marburg Virus, NP, VP35, and L, Are Sufficient To Mediate Replication and Transcription of Marburg Virus-Specific Monocistronic Minigenomes
Northern blot analysis of viral RNA revealed that three nucleocapsid proteins were essential and sufficient for transcription as well as replication and encapsidation and indicate that VP35, rather than VP30, is the functional homologue of rhabdo- and paramyxovirus P proteins.
Inhibition of Marburg virus protein expression and viral release by RNA interference.
The efficient reduction of MARV replication also suggests that RNAi may provide an agent against MHF, and targeted reduction of VP30 downregulated the intracellular levels of all other viral proteins, suggesting that VP30 plays an essential role for transcription/replication.
Interactions of Marburg virus nucleocapsid proteins.
The components of Marburg virus nucleocapsid complex were determined, and interactions between the compounds were investigated, and immunofluorescence analysis of coexpressed proteins was carried out.
Generation of Bovine Respiratory Syncytial Virus (BRSV) from cDNA: BRSV NS2 Is Not Essential for Virus Replication in Tissue Culture, and the Human RSV Leader Region Acts as a Functional BRSV Genome Promoter
The successful recovery of a BRSV mutant lacking the complete NS2 gene, which encodes a nonstructural protein of unknown function is reported, demonstrating that NS2 is not essential for virus replication in cell culture.
Reverse Genetics Demonstrates that Proteolytic Processing of the Ebola Virus Glycoprotein Is Not Essential for Replication in Cell Culture
Generation of a viable mutant Ebola virus lacking a furin recognition motif in theGP cleavage site demonstrates that GP cleavage is not essential for replication of Ebola virus in cell culture.
RNA Polymerase I-Driven Minigenome System for Ebola Viruses
A comparison between minigenomes and helper plasmids from different filovirus species and genera indicates that inherent differences in the transcription and/or replication capacities of the ribonucleoprotein complexes of pathogenic and apathogenic filoviruses may exist, as these observations were confirmed in a Lake Victoria marburgvirus minigenome system.
Infectious rabies viruses from cloned cDNA.
The generation of infectious rabies virus (RV), a non‐segmented negative‐stranded RNA virus of the Rhabdoviridae family, entirely from cloned cDNA is described, and the possibility of manipulating the RV genome by recombinant DNA techniques using the described procedure greatly facilitates the investigation of RV genetics, virus‐host interactions and rabies pathogenesis.