Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling

@inproceedings{CalcagnoPizarelli2011RescueOA,
  title={Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling},
  author={Ana Mar{\'i}a Calcagno-Pizarelli and Am{\'e}rica Herv{\'a}s-Aguilar and Antonio Galindo and Juan F. Abenza and Miguel A Pe{\~n}alva and Herbert N. Arst},
  booktitle={Journal of cell science},
  year={2011}
}
The Aspergillus pal pathway hijacks ESCRT proteins into ambient pH signalling complexes. We show that components of ESCRT-0, ESCRT-I, ESCRT-II and ESCRT-III are nearly essential for growth, precluding assessment of null mutants for pH signalling or trafficking. This severely debilitating effect is rescued by loss-of-function mutations in two cation tolerance genes, one of which, sltA, encodes a transcription factor whose inactivation promotes hypervacuolation. Exploiting a conditional… CONTINUE READING