Rescue of American chestnut with extraspecific genes following its destruction by a naturalized pathogen

@article{Steiner2016RescueOA,
  title={Rescue of American chestnut with extraspecific genes following its destruction by a naturalized pathogen},
  author={Kim C. Steiner and Jared W. Westbrook and Frederick V. Hebard and Laura L. Georgi and William A. Powell and Sara F. Fitzsimmons},
  journal={New Forests},
  year={2016},
  volume={48},
  pages={317-336}
}
Following the near-obliteration of American chestnut (Castanea dentata [Marsh.] Borkh.) by the chestnut blight early in the last century, interest in its restoration has been revived by efforts to develop a blight-resistant form of the species. We summarize progress and outline future steps in two approaches: (1) a system of hybridizing with a blight-resistant chestnut species and then backcrossing repeatedly to recover the American type and (2) transformation of American chestnut with a… 
Intentional introgression of a blight tolerance transgene to rescue the remnant population of American chestnut
TLDR
It will also be critically important that optimal restoration trees should have robust genetic diversity and resilience, which can be supplied by a full complement of their wild‐type genes.
Restoration of the American chestnut will require more than a blight-resistant tree.
The American chestnut (Castanea dentata) was a keystone species that was decimated by nonnative diseases, most notably a fungus (Cryphonectria parasitica) that causes chestnut blight disease, during
Supplemental materials for Beyond blight: Phytophthora root rot under climate change limits populations of reintroduced American chestnut
American chestnut ( Castanea dentata ) was functionally extirpated from eastern US forests by chestnut blight, caused by a fungus from Asia. As efforts to produce blight-resistant American chestnut
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TLDR
Targeted expression of OxO to wounded and infected tissue is sought as an alternative to constitutive expression for potential metabolic resource conservation and transgene stability over the long lifetime of a tree and over successive generations of breeding.
Resurrecting the Lost Flames of American Chestnut
TLDR
The loss of American chestnut may have altered litter flammability of some eastern US forest ecosystems, a result more commonly associated with compositional changes in conjunction with fire exclusion and other disturbances, and Resurrection of lost foundation species through introduction of resistant genotypes may represent a resounding ecological success story, but unanticipated changes to ecological processes, such as fire, should be considered.
Developing Blight-Tolerant American Chestnut Trees.
TLDR
To help restore this important canopy tree, blight-tolerant American chestnut trees have been developed using an oxalate oxidase-encoding gene from wheat.
Transformation of American Chestnut (Castanea dentata (Marsh.) Borkh) Using RITA® Temporary Immersion Bioreactors and We Vitro Containers
TLDR
American chestnut was successfully transformed with a detoxifying enzyme, oxalate oxidase, to enhance blight tolerance and more recently with the Cast_Gnk2-like gene, which encodes for an antifungal protein, to be tested for P. cinnamomi putative tolerance.
“A reference genome assembly and adaptive trait analysis of Castanea mollissima ‘Vanuxem,’ a source of resistance to chestnut blight in restoration breeding”
TLDR
The assembly of a reference genome for Chinese chestnut (C. mollissima) “Vanuxem” is presented, one of the donors of disease resistance for American chestnut restoration, and the value of the genome as a platform for research and species restoration is demonstrated.
Identifying Host Resistance to Phytophthora cinnamomi in Hybrid Progeny of Castanea dentata and Castanea mollissima
Phytophthora cinnamomi Rands, the causal pathogen of phytophthora root rot (PRR) of chestnut, is one of the main obstacles to growth of american chestnut [Castanea dentata (Marsh.) Bork.] in the
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