Rescue In Vitro Fertilization Method for Legacy Stock of Frozen Mouse Sperm

@inproceedings{Nakagata2014RescueIV,
  title={Rescue In Vitro Fertilization Method for Legacy
Stock of Frozen Mouse Sperm},
  author={Naomi Nakagata and Toru Takeo and Kiyoko Fukumoto and Yukie Haruguchi and Tomoko Kondo and Yumi Takeshita and Yuko Nakamuta and Tomoko Umeno and Shuuji Tsuchiyama},
  booktitle={The Journal of reproduction and development},
  year={2014}
}
Sperm cryopreservation has been widely adopted for maintenance of the genetically engineered mouse (GEM). The cryopreserved sperm are being exchanged among many institutes worldwide. However, the recipients are not always able to obtain high fertilization rates with the frozen sperm shipped from senders. In this study, we cryopreserved mouse sperm via various methods and performed in vitro fertilization (IVF) in which the combination of methyl-beta-cyclodextrin for sperm preincubation and… CONTINUE READING