Requirements for the formation of plasmid-transducing particles of Bacillus subtilis bacteriophage SPP1.

@article{Alonso1986RequirementsFT,
  title={Requirements for the formation of plasmid-transducing particles of Bacillus subtilis bacteriophage SPP1.},
  author={Juan Alonso and Gerbild L{\"u}der and Thomas A. Trautner},
  journal={The EMBO journal},
  year={1986},
  volume={5 13},
  pages={3723-8}
}
We had previously proposed that the production of concatemeric plasmid DNA in plasmid-transducing SPP1 particles is a consequence of phage-directed rolling-circle-type replication of plasmid DNA. The production of such DNA was greatly enhanced when DNA/DNA homology was provided between phage and plasmid DNAs (facilitation of transduction). Here we present evidence that synthesis of concatemeric plasmid DNA can proceed after phage infection under conditions non-permissive for plasmid replication… CONTINUE READING

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