Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase.

@article{Lenz2005RequirementsFH,
  title={Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase.},
  author={Oliver Lenz and Andrea Gleiche and Angelika Strack and B. Cornelia Friedrich},
  journal={Journal of bacteriology},
  year={2005},
  volume={187 18},
  pages={6590-5}
}
By taking advantage of the tightly clustered genes for the membrane-bound [NiFe] hydrogenase of Ralstonia eutropha H16, broad-host-range recombinant plasmids were constructed carrying the entire membrane-bound hydrogenase (MBH) operon encompassing 21 genes. We demonstrate that the complex MBH biosynthetic apparatus is actively produced in hydrogenase-free hosts yielding fully assembled and functional MBH protein. 

From This Paper

Figures, tables, and topics from this paper.
8 Citations
57 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 57 references

Analysis of the transcarbamoylation - dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE

  • S. Huth
  • Eur . J . Biochem .
  • 2004

The H2-metabolizing prokaryotes

  • E. Schwartz, B. Friedrich
  • 2003

Similar Papers

Loading similar papers…