Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC7120

@article{Frias1994RequirementOT,
  title={Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC7120},
  author={José E. Frías and Enrique Flores and Antonia Herrero},
  journal={Molecular Microbiology},
  year={1994},
  volume={14}
}
The cyanobacterial ntcA gene encodes a DNA‐binding protein that belongs to the Crp family of bacterial transcriptional regulators. In this work, we describe the isolation of an ntcA insertional mutant of the dinitrogen‐fixing, heterocyst‐forming cyanobacterium Anabaena sp. PCC 7120. The Anabaena ntcA mutant was able to use ammonium as a source of nitrogen for growth, but was unable to assimilate atmospheric nitrogen (dinitrogen) or nitrate. Nitrogenase and enzymes of the nitrate reduction… Expand
Open Reading Frame all0601 from Anabaena sp. Strain PCC 7120 Represents a Novel Gene, cnaT, Required for Expression of the Nitrate Assimilation nir Operon
TLDR
An insertional mutant of cnaT was generated and found to be unable to assimilate nitrate, although it could use ammonium or dinitrogen as a source of nitrogen for growth, and the previously described regulatory elements, NtcA and NtcB, is discussed. Expand
Activation of the Anabaena nir operon promoter requires both NtcA (CAP family) and NtcB (LysR family) transcription factors
TLDR
Results showed that NtcB, a LysR‐ family protein, is required in addition to NtcA, a CAP‐family protein, for the expression of genes encoding proteins specifically involved in nitrate assimilation in Anabaena sp. Expand
Role of NtcA, a cyanobacterial global nitrogen regulator, in the regulation of sucrose metabolism gene expression in Anabaena sp. PCC 7120
TLDR
It is concluded that sucrose metabolism is coordinated at the transcriptional level with nitrogen metabolism, suggesting a global metabolism regulating role for NtcA. Expand
Nitrate assimilation gene cluster from the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120
A region of the genome of the filamentous, nitrogen-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 that contains a cluster of genes involved in nitrate assimilation has beenExpand
Regulation of ntcA Expression and Nitrite Uptake in the Marine Synechococcus sp. Strain WH 7803
TLDR
Nitrite and nitrate act as specific inducers for the synthesis of proteins required for nitrite uptake in cyanobacteria cloned and sequenced, suggesting that ntcA expression is not sufficient for uptake capacity to develop. Expand
The Global Nitrogen Regulator NtcA Regulates Transcription of the Signal Transducer PII (GlnB) and Influences Its Phosphorylation Level in Response to Nitrogen and Carbon Supplies in the Cyanobacterium Synechococcus sp. Strain PCC 7942
TLDR
A good correlation between the amounts of the two transcript species and the PII protein was observed, as revealed by immunodetection with specific antibodies, and the phosphorylation level of PII in the wild type is inversely correlated with nitrogen availability and directly correlated with higher CO2 concentration. Expand
Transcription of glutamine synthetase genes (glnA and glnN) from the cyanobacterium Synechocystis sp. strain PCC 6803 is differently regulated in response to nitrogen availability
TLDR
It is shown that expression of both the glnA and glnN genes is subjected to transcriptional regulation in response to changes in nitrogen availability, and that an additional modification of NtcA or an additional factor is required for the regulation of glnnN gene expression. Expand
NrrA, a nitrogen‐responsive response regulator facilitates heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120
TLDR
It is concluded that the nrrA gene, which encodes a response regulator of the OmpR family with a DNA‐binding domain, facilitates heterocyst development. Expand
Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro
TLDR
It is proposed that the intracellular concentration of 2-oxoglutarate functions as a signaling molecule that transmits information on cellular nitrogen status to NtcA and thereby regulates the transcription of genes related to nitrogen assimilation in cyanobacteria. Expand
Posttranscriptional Regulation of Glutamine Synthetase in the Filamentous Cyanobacterium Anabaena sp. PCC 7120: Differential Expression between Vegetative Cells and Heterocysts
TLDR
In vitro studies demonstrate that Anabaena GS is not inactivated by Synechocystis IFs (IF7 and IF17), indicating the specificity of the system and differential expression of the IF system between heterocysts and vegetative cells of AnabaENA. Expand
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TLDR
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A second nitrogen fixation (nif) operon in the cyanobacterium (blue-green alga) Anabaena (Nostoc) strain PCC 7120 has been identified and sequenced and contains two instances of 7-base pair directly repeated sequences. Expand
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TLDR
It is concluded that internal partial oxygen pressure sufficient to inactivate nitrogenase is insufficient to repress synthesis of the enzyme completely; among mutants with an apparently intact heterocyst envelope and normal respiration, three had virtually undetectable levels of dinitrogenase reductase under all conditions employed. Expand
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TLDR
Sequence analysis has shown that these ntcA genes would encode polypeptides strongly similar (77 to 79% identity) to the Synechococcus NtcA protein. Expand
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TLDR
Results provide evidence for a causal relationship between development and transcriptional events in Anabaena and three lines of evidence indicate that the mutant has retained the ability to develop a pattern despite its inability to make heterocysts. Expand
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Twenty-seven mutants that were unable to assimilate nitrate were isolated from Synechococcus sp. strain PCC 7942. In addition to mutants that lacked nitrate reductase or nitrite reductase, sevenExpand
Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection
TLDR
The bifA gene is cloned by using a genetic selection strategy to identify a 672-bp open reading frame that encoded VF1-like binding activity and shows 77% identity to NtcA, which is a global regulator involved in nitrogen control in Synechococcus sp. Expand
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