Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.

@article{Kline1986RequirementOT,
  title={Requirement of the Escherichia coli dnaA gene product for plasmid F maintenance.},
  author={Bruce C. Kline and Tokio Kogoma and Jeffrey E. Tam and M. S. Megan G. Shields},
  journal={Journal of bacteriology},
  year={1986},
  volume={168 1},
  pages={440-3}
}
There are DnaA protein-binding sites in at least one F origin of replication, and only potentially leaky dnaA(Ts) mutations had ever been used in previous studies indicating that F replication was independent of the dnaA gene product. Here we show that an Escherichia coli dnaA::Tn10 host which does not make a dnaA gene product cannot sustain autonomous or integrated F plasmid maintenance. 

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