Requirement of a plasmid-encoded protein for replication in vitro of plasmid R6K.

@article{Inuzuka1978RequirementOA,
  title={Requirement of a plasmid-encoded protein for replication in vitro of plasmid R6K.},
  author={Manabu Inuzuka and D. R. Helinski},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1978},
  volume={75 11},
  pages={5381-5}
}
Conditions are described for the replication of exogeneous R6K DNA in an in vitro system prepared from Escherichia coli cells. Replication of plasmid DNA in this system is semiconservative and sensitive to actinomycin D, novobiocin, arabinofuranosyl-CTP,N-ethylmaleimide, and inhibitors of DNA-dependent RNA polymerase. An ammonium sulfate fraction prepared from cells carrying the R6K plasmid is required for replication. A direct role in replication for a plasmid-encoded protein, designated pi… CONTINUE READING

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