Requirement for calcium ion in insulin secretion by the perfused rat pancreas.

@article{Curry1968RequirementFC,
  title={Requirement for calcium ion in insulin secretion by the perfused rat pancreas.},
  author={Donald L. Curry and Leslie L. Bennett and Gerold M. Grodsky},
  journal={The American journal of physiology},
  year={1968},
  volume={214 1},
  pages={
          174-8
        }
}
CURRY, DONALD L., LESLIE L. BENNETT, AND GEROLD M. GR~DSKY. Requirement for calcium ion in insulin secretiorz by the perfused rut p ancreas. Am. J. Physiol. 214(l): 174-178. 1968.The effect of calcium ion on insulin release was studied in the isolated perfused rat pancreas. Following a standard glucose stimulus, insulin secretion (determined by immunochemical assay of the total portal vein effluent) was directly dependent on perfusate calcium ion concentrations up to 4 mEq/liter, At calcium… Expand
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An isolated in vitro pancreas perfusion preparation is described which is suitable for study of insulin secretion and demonstrates increased secretion of insulin with tolbutamide and glucose. Expand
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The question obviously arises whether the role of calcium at the adrenal medulla is unique or whether calcium may also be intimately involved in other secretory processes, particularly those which are likewise initiated by ACh. Expand
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VARIOUS techniques have been devised in an effort to study the factors which directly influence insulin secretion. Zunz and La Barre (1927) joined two dogs by a pancreatic-jugular anastomosis andExpand
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The modification of this approach for the assay of circulating lhumiian insulin is the subject of this work, which forms the basis of methods recently described for the measurement of injected insulin in rabbit plasma and extracted insulin from mouse pancreas. Expand
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The effect of carbohydrates on the secretion of immunochemically measurable insulin was studied in an isolated perfused pancreatic preparation from the rat. Degradation of circulating insulin (as m...
A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSE
The reliability of the various Somogyi-Shaffer-Hartmann (1, 2) copper reagents for glucose determination in biological material has been established. Adaptation of these reagents to calorimetric useExpand
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