Requirement for a reduced sulphydryl entity in the protection of molybdate-stabilized estrogen receptor.

The separate and combined effect of molybdate and dithiothreitol on the stability of human uterine 9 S estrogen receptor were studied. Maximal, short-term, protection of the 9 S estrogen receptor was achieved by the joint inclusion of both stabilizing agents in cytosol buffers. This molybdate--dithiothreitol-mediated stability was dependent on reducing… (More)