Requirement for ATP by the DNA damage checkpoint clamp loader.

  title={Requirement for ATP by the DNA damage checkpoint clamp loader.},
  author={Jerzy Majka and Brian H. Y. Chung and Peter M. J. Burgers},
  journal={The Journal of biological chemistry},
  volume={279 20},
The DNA damage clamp loader replication factor C (RFC-Rad24) consists of the Rad24 protein and the four small Rfc2-5 subunits of RFC. This complex loads the heterotrimeric DNA damage clamp consisting of Rad17, Mec3, and Ddc1 (Rad17/3/1) onto partial duplex DNA in an ATP-dependent manner. Interactions between the clamp loader and the clamp have been proposed to mirror those of the replication clamp loader RFC and the sliding clamp proliferating cell nuclear antigen (PCNA). In that system, three… CONTINUE READING