Reprogramming primordial germ cells (PGC) to embryonic germ (EG) cells.

@article{DurcovaHills2008ReprogrammingPG,
  title={Reprogramming primordial germ cells (PGC) to embryonic germ (EG) cells.},
  author={Gabriela Durcova-Hills and Azim Surani},
  journal={Current protocols in stem cell biology},
  year={2008},
  volume={Chapter 1},
  pages={
          Unit1A.3
        }
}
In this unit we describe the derivation of pluripotent embryonic germ (EG) cells from mouse primordial germ cells (PGCs) isolated from both 8.5- and 11.5-days post-coitum (dpc) embryos. Once EG cells are derived we explain how to propagate and characterize the cell lines. We introduce readers to PGCs and explain differences between PGCs and their in vitro derivatives EG cells. Finally, we also compare mouse EG cells with ES cells. This unit will be of great interest to anyone interested in PGCs… CONTINUE READING
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