Reprogramming of human primary somatic cells by OCT4 and chemical compounds.

@article{Zhu2010ReprogrammingOH,
  title={Reprogramming of human primary somatic cells by OCT4 and chemical compounds.},
  author={Saiyong Zhu and Wenlin Li and H. Zhou and Wanguo Wei and R. Ambasudhan and Tongxiang Lin and Janghwan Kim and K. Zhang and Sheng Ding},
  journal={Cell stem cell},
  year={2010},
  volume={7 6},
  pages={
          651-5
        }
}
Induced pluripotent stem cell (iPSC) technology, i.e. reprogramming somatic cells into pluripotent cells that closely resemble embryonic stem cells (ESCs) by introduction of defined transcription factors (TFs), holds great potential in biomedical research and regenerative medicine (Takahashi et al., 2006; Takahashi et al., 2007; Yu et al., 2007). Various strategies have been developed to generate iPSCs with fewer or no exogenous genetic manipulations, which represent a major hurdle for iPSC… Expand
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Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4
TLDR
A small-molecule screening platform applied to identify compounds that functionally replace the reprogramming factor Klf4 gave rise to iPS cells that are indistinguishable from murine embryonic stem cells, and can be used to screen large chemical libraries in search of novel compounds to replace theReprogramming factors that induce pluripotency. Expand
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2
TLDR
Valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4, and supports the possibility of reprograming through purely chemical means. Expand
Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds.
TLDR
A small-molecule combination, BIX-01294 and BayK8644, is identified that enables reprogramming of Oct4/Klf4-transduced mouse embryonic fibroblasts, which do not endogenously express the factors essential for reprograming. Expand
Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes
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It is shown that reprograming of juvenile human primary keratinocytes by retroviral transduction with OCT4, SOX2, KLF4 and c-MYC is at least 100-fold more efficient and twofold faster compared with reprogramming of human fibroblasts. Expand
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It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Expand
Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors
TLDR
Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Expand
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It is reported that a specific glycogen synthase kinase 3 (GSK‐3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. Expand
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This work sought to create a doxycycline-inducible lentiviral system to convert primary human fibroblasts and keratinocytes into human induced pluripotent stem cells (hiPSCs), and developed a strategy to induce hiPSC formation at high frequency. Expand
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,Expand
Vitamin C enhances the generation of mouse and human induced pluripotent stem cells.
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It is reported that a natural compound, vitamin C (Vc), enhances iPSC generation from both mouse and human somatic cells, acts at least in part by alleviating cell senescence, a recently identified roadblock for reprogramming. Expand
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