Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.

@article{Dunne2019ReprogrammingBH,
  title={Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins.},
  author={Matthew Dunne and Beatrice Rupf and Marcelo Tala and Xhem Qabrati and P. Ernst and Yang Shen and Eric. T. Sumrall and Laura Heeb and Andreas Pl{\"u}ckthun and Martin J. Loessner and Samuel Kilcher},
  journal={Cell reports},
  year={2019},
  volume={29 5},
  pages={
          1336-1350.e4
        }
}
Bacteriophages provide excellent tools for diagnostics, remediation, and targeted microbiome manipulation, yet isolating viruses with suitable host specificity remains challenging. Using Listeria phage PSA, we present a synthetic biology blueprint for host-range engineering through targeted modification of serovar-specific receptor binding proteins (RBPs). We identify Gp15 as the PSA RBP and construct a synthetic phage library featuring sequence-randomized RBPs, from which host range mutants… 
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