Reproducible microwave-assisted acid hydrolysis of proteins using a household microwave oven and its combination with LC-ESI MS/MS for mapping protein sequences and modifications.

@article{Wang2010ReproducibleMA,
  title={Reproducible microwave-assisted acid hydrolysis of proteins using a household microwave oven and its combination with LC-ESI MS/MS for mapping protein sequences and modifications.},
  author={Nan Wang and Liang Li},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2010},
  volume={21 9},
  pages={1573-87}
}
A new set-up for microwave-assisted acid hydrolysis (MAAH) with high efficiency and reproducibility to degrade proteins into peptides for mass spectrometry analysis is described. It is based on the use of an inexpensive domestic microwave oven and can be used for low volume protein solution digestion. This set-up has been combined with liquid chromatography… CONTINUE READING