Reproducibility of human sperm DNA measurements using the alkaline single cell gel electrophoresis assay.

@article{Hughes1997ReproducibilityOH,
  title={Reproducibility of human sperm DNA measurements using the alkaline single cell gel electrophoresis assay.},
  author={Carmel M. Hughes and Susan E. Lewis and Valerie J. McKelvey-Martin and Wayne Thompson},
  journal={Mutation research},
  year={1997},
  volume={374 2},
  pages={261-8}
}
The single cell gel electrophoresis (SCGE) assay is a simple visual technique used to assess DNA integrity in individual cells by measuring damage reflected as strand breaks under alkaline conditions. Cells are embedded in agarose on glass slides followed by lysis of the cell membranes after which damaged DNA strands are electrophoresed away from the nucleus towards the anode and deposited to one side giving the appearance of a tail. DNA damage may be measured by assessing the relative amounts… CONTINUE READING
35 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 35 extracted citations

Similar Papers

Loading similar papers…