Reprint of: Making the most of affinity tags.

  • David S Waugh
  • Published 2011 in Protein expression and purification

Abstract

Proteins do not naturally lend themselves to high-throughput analysis because of their diverse physiochemical properties. Consequently, affinity tags have become indispensable tools for structural and functional proteomics initiatives. Although originally developed to facilitate the detection and purification of recombinant proteins, in recent years it has… (More)

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