Repression of retrotransposal elements in mouse embryonic stem cells is primarily mediated by a DNA methylation-independent mechanism.

@article{Hutnick2010RepressionOR,
  title={Repression of retrotransposal elements in mouse embryonic stem cells is primarily mediated by a DNA methylation-independent mechanism.},
  author={Leah K. Hutnick and Xinhua Huang and Tao-Chuan Loo and Zhicheng Ma and Guoping Fan},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 27},
  pages={21082-91}
}
In defense of deleterious retrotransposition of intracisternal A particle (IAP) elements, IAP loci are heavily methylated and silenced in mouse somatic cells. To determine whether IAP is also repressed in pluripotent stem cells by DNA methylation, we examined IAP expression in demethylated mouse embryonic stem cells (mESCs) and epiblast-derived stem cells. Surprisingly, in demethylated ESC cultures carrying mutations of DNA methyltransferase I (Dnmt1), no IAP transcripts and proteins are… CONTINUE READING
42 Citations
2 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 42 extracted citations

References

Publications referenced by this paper.
Showing 1-2 of 2 references

Reprod. Biol. Endocrinol

  • M. A. Ramírez, E. Pericuesta, R. Fernandez-Gonzalez, P. Moreira, B. Pintado, A. Gutierrez-Adan
  • JULY
  • 2006

Retrotransposon IAP Regulation in mESCs and EpiSCs JULY 2, 2010

  • E. L. Kuff, J. W. Fewell
  • Cell Biol
  • 1985

Similar Papers

Loading similar papers…