Reporter proteins for in vivo fluorescence without oxygen

@article{Drepper2007ReporterPF,
  title={Reporter proteins for in vivo fluorescence without oxygen},
  author={Thomas Drepper and Thorsten Eggert and Franco Circolone and Achim Heck and Ulrich Krauss and Jan-Karl Guterl and Marion Wendorff and Aba Losi and Wolfgang G{\"a}rtner and Karl‐Erich Jaeger},
  journal={Nature Biotechnology},
  year={2007},
  volume={25},
  pages={443-445}
}
Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage–sensing domains, we engineered flavin mononucleotide–based fluorescent proteins that can be… Expand
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