Reporter-based assays for analyzing RNA interference in mammalian cells.

Abstract

RNA interference (RNAi) is a process whereby small RNAs serve as effectors to direct posttranscriptional regulation of gene expression. The effector small RNAs can arise from various sources including plasmids that express short-hairpin RNAs (shRNAs) or microRNA (miRNAs), or alternatively, from synthetic small-interfering RNAs (siRNAs). These small RNAs… (More)
DOI: 10.1007/978-1-61779-046-1_12

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