Replicon-helper systems from attenuated Venezuelan equine encephalitis virus: expression of heterologous genes in vitro and immunization against heterologous pathogens in vivo.

@article{Pushko1997RepliconhelperSF,
  title={Replicon-helper systems from attenuated Venezuelan equine encephalitis virus: expression of heterologous genes in vitro and immunization against heterologous pathogens in vivo.},
  author={Peter Pushko and M{\'o}nica Parker and George V. Ludwig and Nancy L. Davis and R. E. Johnston and Jonathan F. Smith},
  journal={Virology},
  year={1997},
  volume={239 2},
  pages={389-401}
}
A replicon vaccine vector system was developed from an attenuated strain of Venezuelan equine encephalitis virus (VEE). The replicon RNA consists of the cis-acting 5' and 3' ends of the VEE genome, the complete nonstructural protein gene region, and the subgenomic 26S promoter. The genes encoding the VEE structural proteins were replaced with the influenza virus hemagglutinin (HA) or the Lassa virus nucleocapsid (N) gene, and upon transfection into eukaryotic cells by electroporation, these… CONTINUE READING
Highly Influential
This paper has highly influenced 25 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
153 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 153 extracted citations

Similar Papers

Loading similar papers…