Replication of white spot syndrome virus in ovarian primary cultures from the kuruma shrimp, Marsupenaeus japonicus.

@article{Maeda2004ReplicationOW,
  title={Replication of white spot syndrome virus in ovarian primary cultures from the kuruma shrimp, Marsupenaeus japonicus.},
  author={Minoru Maeda and Hiroyuki Saitoh and Eiichi Mizuki and Toshiaki Itami and Michio Ohba},
  journal={Journal of virological methods},
  year={2004},
  volume={116 1},
  pages={89-94}
}
Propagation of white spot syndrome virus (WSSV) was investigated in primary ovarian cultures from the kuruma shrimp Marsupenaeus japonicus. A WSSV strain, purified by sucrose density gradient centrifugation, was inoculated into 10-day-old primary ovarian cultures. WSSV infection induced marked cytopathic effect (CPE) on primary ovarian cells. Initially, virus-infected cells began to shrink 72 h post-inoculation, followed by the rounding of most cells which detached finally from flask surface… CONTINUE READING
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