Replication of the linear chromosomal DNA from the centrally located oriC of Streptomyces ambofaciens revealed by PFGE gene dosage analysis.

@article{Fischer1998ReplicationOT,
  title={Replication of the linear chromosomal DNA from the centrally located oriC of Streptomyces ambofaciens revealed by PFGE gene dosage analysis.},
  author={Gilles Fischer and Anne Catherine Holl and Jean Nicolas Volff and Dominique Vandewiele and Bernard Decaris and Pierre Leblond},
  journal={Research in microbiology},
  year={1998},
  volume={149 3},
  pages={
          203-10
        }
}
From a cosmid clone of Streptomyces ambofaciens containing the dnaA and gyrAB genes, a 2.7-kb self-replicating DNA fragment containing the chromosome replication origin oriC was isolated. This cosmid was previously maped physically to a region near the middle of the 8-Mb linear chromosomal DNA. A pulsed-field gel electrophoresis time-course analysis revealed that sequences flanking oriC were overrepresented relative to the rest of the chromosomal DNA during rapid growth, indicating that this… CONTINUE READING
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